Bio-Rad Prep-A-Gene

Patricia Foster pfoster at bu.edu
Sat Mar 26 13:32:58 EST 1994


   We had been using Magic minipreps to prepare E. coli
genomic DNA.  The standard plasmid protocol worked well
enough to give us a good prep for PCRing our 2kb
(unusually difficult) fragment.  Wizard does not work.
We have now tried home-made resin (recipes posted here), Bio-rad
(both protocols for plasmid and genomic DNA) and Gene-
release from BioVentures.  Dismal failures all around.
We are back to phenol/chloroform extractions.
Sigh
Pat
--
Patricia L. Foster
Boston University School of Medicine
Boston, MA USA
pfoster at bu.edu



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