help needed on gel retardation assay
leach at mbcrr.harvard.edu
Sat Mar 26 21:15:56 EST 1994
In article <2mvop3INNcgp at dns1.NMSU.Edu>, twei at kirk (Tao Wei) wrote:
> Dear netters:
> I am a graduate student and working with tomato plant. I am running into
> a problem in gel retardation experiments. The DNA probe (200 bp prepared
> by PCR) and crude nuclear proteins seemed to form very large complexes
> (because of non-specific interaction?), stuck on the bottom of wells and
> could not get into the gel. The condition of binding reaction was pH 7.5,
> 50 mM NaCl, 9-11% glycerol and some competitor DNA. Does anybody have any
> suggestions to help me out? Thank in advance.
How much protein??? Possibly too much!!
Try cutting down on the protein!
..... Martin Leach Email:leach at mbcrr.harvard.edu
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