Northern Problem

MALBOOBI at QUCDN.QueensU.CA MALBOOBI at QUCDN.QueensU.CA
Sat Mar 26 20:11:07 EST 1994


1. Have you purified your probe from unincorporated dNTPs? I used Clontech Spun
column-30 to do so.
2. You may consider your the volume of the washing solutions and the amount of
time for each wash. I recommend at least 300 ml and 30 min.
If you have further question, please send me a mail. I can send you the protcol
es that I used to do several N. blot.
Good Luck
M.A. Malboobi



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