baculovirus and PCR

Shiao Y. Wang sywang at whale.st.usm.edu
Sun Mar 27 23:08:31 EST 1994


I have a pair of primers that I use to detect baculovirus infected shrimp.
The problem is that the PCR reaction doesn't always work. Since
baculovirus causes that production of occlusion bodies (protein crystals
containing virions), one possible reason is that simply boiling the
infected tissue in water does not release the DNA for amplification. I
would be grateful for information on how you extract DNA from tissues for PCR.



More information about the Methods mailing list