mkennedy at chmeds.ac.nz
Sun Mar 27 22:21:27 EST 1994
In article <0097BF8A.E8C25A59.789 at BSCR.UGA.EDU>, mcgraw at BSCR.UGA.EDU writes:
> We've got a really weird microsatellite (dinucleotide repeat) marker.
> It's made from a nice-looking CA repeat, and we get some perfectly normal-
> looking products, BUT... In the animals that we've looked at, we see
> either one allele or nothing. In those animals showing one allele, we
> see the various sizes that we would expect from a polymorphic micro-
> satellite, but we never see a heterozygote. It's not just the boys or just
> > the girls. Has anyone out there seen something like this? Any speculations
> on what it might be?
> Al McGraw
Try looking at Koorey et al., 1993. Human Molecular Genetics 2, 289-291. They
found allele non-amplification, often due to a single base substitution at the
3'-end of one of the primers, caused this sort of effect. Try making a new
primer at a different site.
NNNN NN Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) ZZZZZZZ
NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ
NN NN NN Christchurch School of Medicine ZZZ
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