Glycogen v tRNA and OLD BONES

Scrimshaw, Brian J SCRIMSHAWB%GRIEHFS at LINCOLN.CRI.NZ
Mon Mar 28 20:16:49 EST 1994


Can someone in netland explain the relative virtues of glycogen
and tRNA as a carrier? Are they just as good as each other for
sticking to DNA?

I'm trying to extract DNA from old bones and have been using
glycogen as a carrier, with little luck, it may just be the DNA
in the bones is too degraded or a protocol problem. I had no luck
with silica and am trying a proteinase K approach with
concentration in a microcon 30 after addition of glycogen, any
advice appreciated.

Brian
Scrimshaw
Wellington, New Zealand



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