Sequencing gel combs (leakage)

the End jgraham at bronze.ucs.indiana.edu
Mon Mar 28 18:55:29 EST 1994


Hello comrads,

Has anyone figured out why sequencing gels will leak on many occasions
allowing loaded samples to flow into adjacent spaces ? I use a "shark's
tooth" comb and more than half of the time find movemnet of the laoded
sample between the plate and the comb.

I often allow my gels to polymerize merely several hours while others
swear that overnight is necessary. This may lead to a soft edge at the 
top perhaps. Otherwize swelling and seperation of the gel from the 
plate may be occurring during the pre-run.

Any tips greatly appreciated. At this point I would just a soon load 
into regular analytical wells due to this problem.


thanks,

Jim

E-mail copies if possible




More information about the Methods mailing list