SV-40 promoter
L.Y.Chau
lyc at IBMS.SINICA.EDU.TW
Mon Mar 28 03:52:37 EST 1994
Hi,
I am doing transfection exp. on undifferentiated and DMSO-induced
differentiated HL-60 cells. The B-gal activity driven by SV-40 promoter is
always 3-fold lower in differentiated HL-60. Here comes the question! Is
SV-40 promoter down-regulated in differentiated cells? If I need to
normalize my transfection data, is there any other promoter I can use which
is not regulated during differentiation?
Jonghwei
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