probe cleaning

B Stickney sticknbd at miranda.cc.vanderbilt.edu
Mon Mar 28 15:27:04 EST 1994


I use Boehringer Mannheim's random primed kit to make DNA probes
for Northerns.  The kit says a Sephadex G-50 column is not necessary
to clean up the probe.  However, I don't see how you'd properly evaluate
your probe in a scintillation counter without running it through a column.
Also, what would be the consequence of adding a bunch of unincorporated
32P-labelled dCTPs to a blot?  It seems to me G-50 or something like it
absolutely is necessary.  Any ideas?
sticknbd at miranda.cc.vanderibilt.edu



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