Serum rich medium for lymphocyte cultures- any recipes?

Marion Freistadt mfreis at lsumc.edu
Mon Mar 28 13:55:11 EST 1994


>Dear Netters,
>
>We are dealing with lymphocyte (t cell) cell cultures and are using media
>prepeared with 10% serum, rest RPMI (as usual).
>This media may be sufficient for short time cultures, but the question
>arises whether they REALLY are sufficiant for long time cultures too. (stuff
>deleted)
>Kurt Schaudt
>
>Medical School of Tuebingen
>
>Germany
>
>kschaudt at mailserv.zdv.uni-tuebingen.de

The ATCC told me that anything that grows in RPMI can grow (and may do
better) in Iscoves, primarily because the buffering capacity of HEPES is
superior.
You still have to add serum or whatever, though.




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