mice heart cultured lines

Marion Freistadt mfreis at lsumc.edu
Mon Mar 28 13:41:52 EST 1994


>
>>        I am looking for a mice heart cell line, more specifically a cardiac
>>muscle cell line.  I looked up the catalog from American Type Culture
>>Collection
>>, and I couldn't find one.  The closest one is a rat heart cell line
>>[H9C2(2-1)]
>>, but it behaves like a skeletal muscle, so I am not sure whether I shall us
e
>>it
>>.
>>        I will greatly appreciate your help and any suggestion on this matte
r.
>
>>michael
>>mman at molbio.cbs.umn.edu

Look up the work from Nick Lanson, William Claycomb, Joe Del Carpio and
Lauren Field. 
They use atrial myocytes from transgenic mice that have an atrial-specific
promotoer driving the SV40 T Ag.  They have developed culture conditions
where the cells beat in culture, and express atrial-specific markers.  They
have carried the lines for four years.





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