Blobs on 35S sequencing gels
Zophonias Oddur Jonsson
zjons at rhi.hi.is
Mon Mar 28 12:33:02 EST 1994
In <2m6vdb$l01 at mercury.hgmp.mrc.ac.uk> mrhodes at crc.ac.uk (Dr. M. Rhodes) writes:
>We have been carrying out 35S sequebcing reactions and have hit a problem
>WE keep getting a "Blob" not a band but definately a blob approx 1cm accross
>going across the whole gel where samples are loaded. It appears to be present
>when you just load the pure 35S-dATP.
Sounds like a buffer problem to me. We had a similar problem when we ran gels
cast with a batch of TBE with a slightly different pH from the buffer that we
used to run the gels.
Zophonias O. Jonsson
>thanks in advance
>email: mrhodes at crc.ac.uk
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