Blobs on 35S sequencing gels

[Zophonias Oddur Jonsson]

In <2m6vdb$l01 at mercury.hgmp.mrc.ac.uk> mrhodes at crc.ac.uk (Dr. M. Rhodes) writes:

>Help
>We have been carrying out 35S sequebcing reactions and have hit a problem
>WE keep getting a "Blob" not a band but definately a blob approx 1cm accross
>going across the whole gel where samples are loaded. It appears to be present 
>when you just load the pure 35S-dATP. 

Sounds like a buffer problem to me.  We had a similar problem when we ran gels
cast with a batch of TBE with a slightly different pH from the buffer that we
used to run the gels.  

Good luck
Zophonias O. Jonsson

>thanks in advance 
>Michael Rhodes
>email: mrhodes at crc.ac.uk