Matchmaker 2 hybrid system

[Carol Scaramuzzi]

Dear Netfolk

I am interested in using the Matchermaker Two hybrid system to investigate
protein-protein interactions between E.coli proteins. This sytem reportedly
works well for eukaryotic genes and their products but I have no
evidence/information concerning the expression of prokaryotic genes. Can
anyone help!. That is, has anyone used this system for bacterial proteins
or have an opinion as to the likely outcome. 

Cheers Scazi