RNA Gels...

[Hanson Lab]

In article <2n2ha1$39c at quartz.ucs.ualberta.ca>,
asimmond at gpu.srv.ualberta.ca (Andrew Simmonds) wrote:

> I am sure this has been covered before....
> I am having trouble getting TIGHT bands with Northern blots from 
> formaldehyde RNA gels...  When I cut off the ladder from the gel and look 
> at it sideways the bands are "tilted" i.e. ( / )   

Sounds to me like your gel comb isn't vertical when you start.  I have seen
much the same with DNA gels when the comb leans forward or back.  
Claudia aka cas9 at cornell.edu