RNA Gels...

Hanson Lab Lab_Mac_Hanson at QMrelay.mail.cornell.edu
Mon Mar 28 09:34:12 EST 1994


In article <2n2ha1$39c at quartz.ucs.ualberta.ca>,
asimmond at gpu.srv.ualberta.ca (Andrew Simmonds) wrote:

> I am sure this has been covered before....
> I am having trouble getting TIGHT bands with Northern blots from 
> formaldehyde RNA gels...  When I cut off the ladder from the gel and look 
> at it sideways the bands are "tilted" i.e. ( / )   

Sounds to me like your gel comb isn't vertical when you start.  I have seen
much the same with DNA gels when the comb leans forward or back.  
Claudia aka cas9 at cornell.edu



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