Double stranded DNA sequencing

Stephen R. Lasky Stephen_Lasky at brown.edu
Mon Mar 28 08:53:08 EST 1994


In article <CnCMoH.CMp at cc.umontreal.ca>, okellyc at megasun.BCH.UMontreal.CA
(Charles J. O'Kelly) wrote:

> In article <Stephen_Lasky-270394110423 at tonto-slip12.cis.brown.edu>, Stephen_Lasky at brown.edu (Stephen R. Lasky) writes:
> |> Try using Promega's Magic Mini-prep columns (or whatever they are calling
> |> them now).  We get 100% sequencable DNA with these.  Fast, easy and give
> |> readable sequences.  
> 
> They're calling them "Wizard PCR preps" now ... and associated with the name change is a major
> change in the composition of the resin.  Be careful.  We've found with ca. 600 bp PCR products
> that the column "eats" about 200 ng of the loaded DNA.  If your prep has less DNA than this ...
> (the "Magic" formula gave better yields in our hands).
> 
> Charley O'Kelly
> Mad Protistologist, neophyte mol biologist
> okellyc at bch.umontreal.ca

-- 
It seems like a lot of companies have recently changed their products for
the worse.  We can't get the TACloning kits to work anymore, the cycle DNA
cDNA synthesis kit used to be great, but then they took out the methyl
mercury and made it the same as all the other cDNA kits.  Now the Magic to
Wizard switch.  Did they do away with the mini-prep kits altogether, or is
this the new version of what was called the Magic PCR clean-up kit?  I
didn't have great luck with that to begin with.  Seemed like it was a
different animal from the Mini-prep kit ( of course its a different type of
DNA that it was supposed to work with).
***************************************************************
Stephen R. Lasky, Ph.D.       Brown University/Roger Williams Medical
Center
e-mail: Stephen_Lasky at brown.edu         LandLine: 401-456-6572
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A nuclear war could ruin your whole day.
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