Blobs on 35S sequencing gels

Martin Leach leach at mbcrr.harvard.edu
Mon Mar 28 23:57:34 EST 1994


In article <2m6vdb$l01 at mercury.hgmp.mrc.ac.uk>, mrhodes at crc.ac.uk (Dr. M.
Rhodes) wrote:

> Help
> We have been carrying out 35S sequebcing reactions and have hit a problem
> WE keep getting a "Blob" not a band but definately a blob approx 1cm accross
> going across the whole gel where samples are loaded. It appears to be present 
> when you just load the pure 35S-dATP. 
> Could it be 
> a) the gel we use National Electrophoresis Rapid gel
> b) heating at 75 degrees in formamide
> c) anything


Hmmm. tuff one. I would hazard a guess at C.
-- 

.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329         
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