Blobs on 35S sequencing gels
leach at mbcrr.harvard.edu
Mon Mar 28 23:57:34 EST 1994
In article <2m6vdb$l01 at mercury.hgmp.mrc.ac.uk>, mrhodes at crc.ac.uk (Dr. M.
> We have been carrying out 35S sequebcing reactions and have hit a problem
> WE keep getting a "Blob" not a band but definately a blob approx 1cm accross
> going across the whole gel where samples are loaded. It appears to be present
> when you just load the pure 35S-dATP.
> Could it be
> a) the gel we use National Electrophoresis Rapid gel
> b) heating at 75 degrees in formamide
> c) anything
Hmmm. tuff one. I would hazard a guess at C.
..... Martin Leach Email:leach at mbcrr.harvard.edu
_|____ Dept. of Pharmacology Phone: (617) 638-5323
/ o / Boston Univ. School of Med. Fax: (617) 638-4329
_/ |-/__==/ 80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118 "Not the old underpants on your
USA head.....WIBBLE" -BLACKADDER
More information about the Methods