leach at mbcrr.harvard.edu
Mon Mar 28 23:56:01 EST 1994
In article <6321.lyc at ibms.sinica.edu.tw_POPMail.PC_3.2.2>,
lyc at IBMS.SINICA.EDU.TW ("L.Y.Chau") wrote:
> I am doing transfection exp. on undifferentiated and DMSO-induced
> differentiated HL-60 cells. The B-gal activity driven by SV-40 promoter is
> always 3-fold lower in differentiated HL-60. Here comes the question! Is
> SV-40 promoter down-regulated in differentiated cells? If I need to
> normalize my transfection data, is there any other promoter I can use which
> is not regulated during differentiation?
On a similar note,
When transfecting into chick primary cortical neuronal cultures with
SV40-luc construct as compared to neuronal promoter-luc constructs i found
the SV40 construct to have higher activity......However, when same cultures
but instead from rat i found the reverse......
That SV-40 prom..... is a funny puppy!
BTW how can a promoter be down-regulated, do you mean the factors to drive
it are not present in some cell-types!!! Maybe so.
p.s. try BioMOO (virtual biology on the internet - telnet
..... Martin Leach Email:leach at mbcrr.harvard.edu
_|____ Dept. of Pharmacology Phone: (617) 638-5323
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