Reverse Transcriptase Question

Warren Gallin wgallin at gpu.srv.ualberta.ca
Mon Mar 28 21:10:24 EST 1994


In Article <940328221804_72170.3155_HHB97-1 at CompuServe.COM>,
72170.3155 at COMPUSERVE.COM (Life Technologies Tech-Line) wrote:
>Does anyone know if mMLV RT or AMV RT will reverse transcribe the cap 
>structure at the 5' end of messages?

It seems hughly unlikely, since the cap G is added in the opposite direction
to the message, i.e. it is linked 5' to 5' with more than one phosphate
group.  That should not act as template.
Warren Gallin,
Department of Zoology, University of Alberta
wgallin at gpu.srv.ualberta.ca



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