Re. Diff.display mRNA borvar@unixg.ubc.ca

babalola at MAIL.SAS.UPENN.EDU babalola at MAIL.SAS.UPENN.EDU
Mon Mar 28 22:43:13 EST 1994


I use DD-RT/PCR with satisfactory signal to noise ratio. I have been
cloning genes off it also. Here are my suggestions:

1. You may be swamping the system with too much RNA starting material.
2. You may be using too much primers.
3. If these guesses are correct, then what you need to do is work out the
optima.
4. Also, you may need to watch out for the right RT and PCR conditions -
i.e. annealing temp., etc.
5. Good luck. E-mail me directly if further problems.


Dr. Lanr Babalola]
University of Pennsylvania
Philadelphia, PA 19104




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