PCR-sequencing

schoenbaechl at urz.unibas.ch schoenbaechl at urz.unibas.ch
Tue Mar 29 20:06:34 EST 1994


Hi everybody,

I am planning to sequence a 1 kb piece from human DNA. I want to use a ds-DNA
PCR sequencing kit from Gibco. Do I first have to amplify this fragment or can
I PCR-sequence directly from DNA isolated from blood ?

I would appreciate hearing about your experiences or suggestions.

		Thanks a lot!

		Mark 



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