Sequencing gel combs (leakage)

Ludwig Lab-UCSC niflab at biology.UCSC.EDU
Tue Mar 29 16:35:14 EST 1994

I have found that loading a little pure blue dye into every fifth
lane sets up a block that stops the leak.  The other four must be 
loaded fairly fast, but as the second lane hits the bottom of the well
(being fully dense) it shoves the leakage up against the comb, where it
does no damage to the resulting autorad.
		--b0b kuhn

Robert Kuhn, PhD              "Twenty years of schoolin'
Sinsheimer Laboratories        and they put you on the day shift
University of California    
Santa Cruz, CA 95064  USA      (brown shoes don't make it)"
                                           -F. Zappa (RIP) 
email: rkuhn at

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