triton-X 1OO in sequencing reaction??

[URA1335 at ARTHUR.CITI2.FR]

Dear netters,

I read a chain termination sequencing
protocol which uses microliter Triton
X-100 in 17 micolt. reaction volume?

I have to questions:

is it usefull?

if yes, which trito X-100 you use?

Because sigma catalogue shows many of them.

Thank you for your answers

ARAL

URA1335 at citi2.fr