mRNA extraction kit

[Nathalie Landry]

In article <yrbois-270394152919 at 128.231.220.135>, yrbois at helix.nih.gov
(Y.R. Boisclair) wrote:

> I want to obtain very good quality mRNA from cells.  I will start from
> small amounts of total RNA (30-100 microgram).  I am debating between the
> micro mRNA purification kit from pharmacia and the Polytract mRNA isolation
> system from Promega.  Which one should I use for good yield and quality ? I
> would like to hear from anybody who has used either system.
> 
> Yves Boisclair
> 
> yrbois at helix.nih.gov

I've tried the mRNA purification kit from Pharmacia and I got some
bad results.  Every time I got a large part of genomic DNA even if I follow
the instructions very strictly.  I would rather try the magnetic system of
Promega or Dynabeads.  I have a beter feeling about it.

Nathalie Landry

nlandry at campus.ulaval.ca