reverse transcriptase question

Dr. C. Dolphin cdolphin at crc.ac.uk
Wed Mar 30 07:47:16 EST 1994

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Article 11471 asks -

Does anyone know if mMLV RT or AMV RT will reverse transcribe the cap
structure at the 5' end of messages?

Yes - according to Hirzmann et al NAR (1993) 21(15) 3597. Haven't tried
it myself though.
Colin

Colin Dolphin				Email: c.t.dolphin at qmw.ac.uk
Biochemistry				Tel: (44) 71 975 5555
Queen Mary & Westfield College	FAX: (44) 81 983 0531
London E1 4NS, UK

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