RNA protection assays

Richard H. Smoke hmhz at umich.edu
Wed Mar 30 14:13:17 EST 1994

  I am about to begin a project involving RNA protection assays and I am
going to use an Ambion kit for both the assay and the invitro
transcription. I have been told to gel purify the the invitro transcription
but could I use a column instead? It seems it would be alot easier. Any
suggestions will be appreciated. I am provided with the probes but I have
to make the RNA myself. Feel free to email me.

Steve Coon
sdcoon at umich.edu
University of Michigan Medical Center

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