Double stranded DNA sequencing
Peter Gegenheimer
peterg at rnaworld.bio.ukans.edu
Wed Mar 30 12:38:20 EST 1994
In <Stephen_Lasky-280394084514 at tonto-slip3.cis.brown.edu>, Stephen_Lasky at brown.edu (Stephen R. Lasky) writes:
>--
>It seems like a lot of companies have recently changed their products for
>the worse. ...... Now the Magic to
>Wizard switch. Did they do away with the mini-prep kits altogether, or is
>this the new version of what was called the Magic PCR clean-up kit?
The "Magic" resin was removed from the market because it was an illegal infringement
on a patent held by BioRad. The original material is BioRad's "Prep-a-Gene".
BioRad also makes an InstaGene resin for preparing PCR-able DNA from cell
lysates. We haven't used these - tried Prep-A-Gene once and it worked, but not any
better than other methods. We always do PCR on bacterial plasmids from half a colony
resuspended directly in the PCR reaction tube; works fine.
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| Peter Gegenheimer | pgegen at kuhub.cc.ukans.edu |
| Departments of Biochemistry and of Botany | voice: 913-864-3939 |
| University of Kansas | FAX : 913-864-5321 |
| 2045 Haworth Hall | "The sleep of reason produces |
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