ashok at biochemistry.cwru.edu
Wed Mar 30 07:51:02 EST 1994
In article <9403300752.AA21396 at cscgpo.anu.edu.au> Klaus.Matthaei at ANU.EDU.AU writes:
>>In a previous article, brett at BORCIM.WUSTL.EDU () says:
>>>>... several people on the methods-and-reagents at net.bio.net
>>>>usenet group have expressed interest in obtaining Klentaq. Any plans for
>>>>distribution? Brett Lindenbach - brett at borcim.wustl.edu
>>KlenTaq is essentially idential to Delta Taq v. 2.0, which is sold by USB.
>>It's cheap, works like a charm, and has replaced Taq as the PCR enzyme of
>>choice in my lab.
>Will Delta Taq v 2.0 PCR a 35Kb fragment for me??
KlenTaq might be the same as DeltaTaq 2.0. However KlenTaqLA --
the enzyme used in the Barnes PNAS paper is a mixture of two
enzymes -- KlenTaq1 and Pfu.
If you added Pfu or Vent or some other error correcting
thermophilic polymerase to DeltaTaq 2.0, it is very likely to
work for long PCRs.
Ashok Aiyar Mail: ashok at biochemistry.cwru.edu
Department of Biochemistry Tel: (216) 368-3300
CWRU School of Medicine, Cleveland, Ohio Fax: (216) 368-4544
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