Glycogen v tRNA and OLD BONES

Uttam Rajbhandary bhandary at wccf.mit.edu
Wed Mar 30 17:04:00 EST 1994


In article <9403291318.4d9781ff.LN1 at Lincoln.cri.nz>, SCRIMSHAWB%GRIEHFS at LINCOLN.CRI.NZ ("Scrimshaw, Brian J") writes...
>Can someone in netland explain the relative virtues of glycogen
>and tRNA as a carrier? Are they just as good as each other for
>sticking to DNA?
> 

I'd use the tRNA.  I have a good feel for recovery percent for, say 5-10 ug of 
carrier tRNA.  Although I've seen references for the use of glycogen, I 
haven't seen any comparisons.  OTOH, will the presence of the tRNA interfere 
with subsequent steps (pcr); certainly, use E.coli tRNA, not calf liver.





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