Tony Sanchez tsanchez at
Thu Mar 31 14:53:22 EST 1994

I am looking into purchasing a PFGE setup.  In the literature there seems to be a 
preference for CHEF.  Is there any basis for this preference, or is it just due to 
familiarity with Bio-Rad and word of mouth.  Has anyone used ROFE?  In 
Methods in Molecular Biology Vol. 12  there is a chapter on ROFE, and it appears 
to be superior to CHEF in my opinion.  It has more field angles and the same or 
better resolution.  It has the advantage of a larger gel (20 x 20 cm) and a small 
footprint (only 25 x 40 cm).  It also does not need a refrig. or cold room.  The main 
draw back I can figure out is that the mechanical rotator is not precise with a short 
pulse time (a few seconds or less).  Does an have any experience using ROFE?  Are 
there other manufactures than Biometra?  How reliable is the rotator?  

Tony Sanchez
University of Florida  Dept. Ophthalmology  1600 SW Archer Road
JHMHC #100284  Gainesville, FL 32610
Phone (904)392-2720      Fax (904) 392-7839           tsanchez at

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