"replacing" SDS with Triton X100

Walt Lilly C749SCB at SEMOVM.SEMO.EDU
Thu Mar 31 10:25:02 EST 1994


We are using an electrophoretic technique in which we separate
enzymes in SDS polyacrylamide gels (without denaturing them first).
Following electrophoresis the enzymes are not active; however, if
we equilibrate the gel with 2.5% triton X100 the activity is restored.
Can anyone explain what is happening here?  Is the triton actually
"replacing" the sds or is it stripping it away into micelles or is
there some other explanation?

Thanks in advance for your insight.

Walt Lilly
c749scb at semovm.semo.edu



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