non-vertical bands on agaorse gels...

rhubner at molbiol.ox.ac.uk rhubner at molbiol.ox.ac.uk
Thu Mar 31 11:26:41 EST 1994


hi there,
 the band positions you describe for your gels are caused by a gradient in
buffer components (i.e. formaldehyde diffusing out of gel)... there is a (huge!)
paper in ANALYTICAL BIOCHEMISTRY 94 (probably January or maybe February issue)
discussing the topic and proposing reformulated buffer solutions to alleviate
the problem ...
best wishes for your northern,
Roland




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