Drying of PAGE gels - Cellophane
Virginia Dress
Dress at biosci.arizona.edu
Thu Mar 31 22:38:12 EST 1994
In article <2nf2p7$ghd at senator-bedfellow.MIT.EDU>, alfken at athena.mit.edu
(Jens Alfken) wrote:
>
>
> Hi,
>
> We are drying a lot of PAGE gels wrapped in cellophane. The
> cellophane we buy currently from BioRad and it is imho very
> expensive. So I went to the local arts supply shop and bought
> a large roll of cellophane for a fraction of the price. Unfortunately
> the stuff doesn`t work ! It is impossible to prewet with water
> (would MeOH/water make a difference ? just a thought). And when
> used the way it is the gel won't dry. It seems the stuff is
> waterimpermeable.
(stuff deleted)
> I guess someone, somewhere must have looked for a replacement
> for the expensive Biotech companies stuff. So what do I have to
> look for ? Any comments wellcome.
>
> Jens Alfken
> alfken at athena.mit.edu
We got a gigantic (5lb roll) of cellophane that we use for drying down
gels.
It lasted from 1990 through all of 1993. We paid $6.25 for the roll
($1.25/lb).
Here's the vendor info:
FLEXEL, Inc
US Highway, 136 West
Covington, Indiana 47932
Cat. # 505848 put-76 untreated
cellophane, type 128
Sorry, no phone number
We soak the cellophane in 10% acetic acid and 20% Ethanol. I've never
tried
soaking it in water. I'm sure the destain for the gel would also work to
wet
the cellophane.
Good luck,
Virginia
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