Methods March 1994 Archives by date

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Starting: Tue Mar 1 00:28:47 EST 1994
Ending: Thu Mar 31 23:01:54 EST 1994
Messages: 1004

LMP agarose gels run in TAE buffer (used to be: Purification from Low Melt) Bernard Murray
LMP agarose gels run in TAE buffer (used to be: Purification from Low Melt) David Micklem
Enzymes for PCR tsanchez at eye1.eye.ufl.edu
making antibodies to low antigenicity proteins Richard Cooke
Densitometry of gels and immunoblots mhollowa at epo.som.sunysb.edu
primer design Alexander Fedorec
inducible promoters William W.-G. Jia
inducible promoters William W.-G. Jia
Quick Hybridizations? Stephen Morris
Nonradioactive labeling(Peroxidase or AP..) Kieron Des
weird Northern Andrew C Palmer
spotty blots Kieron Des
in situ...help Kieron Des
Genuis System Kieron Des
High Efficiency Transformation (library) William J. Buikema
southern blot Kieron Des
Vital dyes Kathie Hodge
What is the gt in lambda gt10 or gt11? Paul Riggs
Insta Preps Reference dkim at unm.edu
Methds-Reagnts archive search? Robert Roxby
Coomassie staining Cornelius Krasel
inducible promoters gc
Shelf life of DTT (Dithiothreitol) Diana Ralston
nucleotide sequence searching via the Internet Kanade Shinkai
Help: Need anti-GAL4 antibodies Gabrielle E. Rieckhof
DNA probe/PCR primers for X,Y Chromosomes Martin Kennedy
Cell culture-transfection-reporter gene: Suggested Readings? Martin Kennedy
What is the gt in lambda gt10 or gt11? Martin Kennedy
water floats on oil? Nicola Collie
nucleotide sequence searching via the Internet Prasit Palitpolkanpim - GRSO
Shelf life of DTT (Dithiothreitol) FRIST at wvnvm.wvnet.edu
BioRad Rotofor Michael J. Studdert
ligation temp.. micjms at lure.latrobe.edu.au
microbiology newsgroup micjms at lure.latrobe.edu.au
DNA quantitation method wanted marian at mbcl.rutgers.edu
Quick Hybridizations? Allison Hanly
inducible promoters Graham Atherton
Fermenter_Wanted_UK bss032 at vaxa.bangor.ac.uk
Fermenter_Wanted_UK bss032 at vaxa.bangor.ac.uk
cDNA libraries Robert B. Darnell
inducible promoters norm eberhardt
amersham's fast hy buffer suter at VAX.MPIZ-KOELN.MPG.d400.de
Genuis System James N Petitte
DNA amplification from silver-stained gels Andreas Vogel
Plasmid extraction micjms at lure.latrobe.edu.au
Methds-Reagnts archive search? Brian Foley
Bicinchoninic Acid (BCA) protein assay Guy.Mordret
no product, Promega kit? Marcella E Grebus
What is the gt in lambda gt10 or gt11? Hank Seifert
Methds-Reagnts archive search? DELLE at IMICILEA.CILEA.IT
no product, Promega kit? Greg Gloor
SOE or Megaprimer PCR mutant libraries ? the End
help to buy aminopropane sulfonic acid Santhosh Kumar
BCA Protein Assay Shaun D. Black
Non-Specificity for Primer Extension ? LIU at QUCDN.QueensU.CA
primer design Paul St. Amand
BCA protein assay (reformatted!) Shaun D. Black
help to buy aminopropane sulfonic acid STASCA at VTHVAX.TAMU.EDU
UNSUBSCRIBE WILLARD at MVAX.WISTAR.UPENN.EDU
nucleotide sequence searching via the Internet Curt Ashendel
nucleotide sequence searching via the Internet Marion Freistadt
Nonradioactive vs. radiolabelled probes Marion Freistadt
NO assays Heather G. Kuruvilla
microbiology newsgroup michael breedveld
Sequencing phenomenon Andrea M Skantar
In vitro Transcription/Translation 37_527 at uwovax.uwo.ca
Catrimox-14 for RNA isolation? Richard R. Hardy
Alas, poor "magic"! I knew thee well. neale at mbcf.stjude.org
SULFINIC acid not sulfonic Santhosh Kumar
Short changed by USB...Taquence sequencing kit Richard J. Hughes
Non-Specificity for Primer Extension ??? LIU at QUCDN.QueensU.CA
A problem in metabolic radiolabelling Hinayana Bawagan
subscription Patricia.Moor at ANU.EDU.AU
Dialog/Paperchase... alvarez at mbcf.stjude.org
Coomassie staining newittja at delphi.com
BioRad Rotofor newittja at delphi.com
SOUTHERNS frances at matai.vuw.ac.nz
NO assays Santhosh Kumar
quick hybridizations Estelle Hrabak
Molecular "tools" micjms at lure.latrobe.edu.au
Specific antibody to Clostridium tetani Prasit Palitpolkanpim - GRSO
Coomassie staining Przemko
test-Don't read this Dick Beeman
Nonradioactive vs. radiolabelled probes M K Bennett
Silver staining with ampholytes LYNDON at BMS-UNIT.ICR.AC.UK
M13 ssDNA-elctrphrtic species?? Dick Beeman
anti-fetal haemoglobin Mr. A.R. Chandra
Guidelines for GST fusion Jason Lei
Spirulina RE - THANKS Dmitry A. Los
Experience with BioRad Rotofor system Andrew, Tel. +39-6-91093434
Nonradioactive vs. radiolabelled probes Wasun Chantratita
Looking for T. Yager JOHN
metabolically labeled nucleotides Dean R. Appling
microbiology newsgroup the End
Catrimox-14 for RNA isolation? RICHARD P. PHIPPS
no product, Promega kit? the End
Shelf life of DTT (Dithiothreitol) Andre-Denis G Wright
IL 1 beta convertase Nigel Appleton
Bicinchoninic Acid (BCA) protein assay Dean Danner Lab
Nonradioactive vs. radiolabelled probes James N Petitte
Short changed by USB...Taquence sequencing kit Will Fischer
plasmids pK19 and pBGS19 KRUEGER, CHARLES M
PROBLRMS WITH BIO-RAD SEQUI-GEN P Watson
vector pAT153? Lakshman Ramamurthy
Catrimox-14 for RNA isolation? douglas l feinstein
Antibodies to CD14 douglas l feinstein
DNA analysis programs Dave Bates
mitoplasts - large scale Dean R. Appling
DnaseI footprinting on single-stranded oligonucleotides Janet Yee
Larval/adult fly expression library needed Joe Kramer
Protein Overexpression in Yeast Joe Kramer
DNA FROM OLD SAMPLES James D DeVault
Wizard & Sequencing Seth Daniel Crosby
Blotter Shiao Y. Wang
Sequencing phenomenon Brian Foley
Quantifying minute amounts of DNA? Virginia Dress
seek antibody to HTLV-1 Basavaraju Shankarappa
anti-rat's endothelium antibodies Paula K Yu
DNA analysis programs Klaus.Matthaei at ANU.EDU.AU
<None> Martin Kennedy
determining oligomeric structure of a protein Roland J Saldanha
Experience with BioRad Rotofor system JOHN PROUDMAN
Restriction map of pAM120 Joe Formanek
Sequencing phenomenon Estelle Hrabak
microbiology newsgroup Joe Formanek
DNA sonication Patricia.Moor at ANU.EDU.AU
DNA analysis programs Rick Hershberger
anti-rat's endothelium antibodies Paula K Yu
DNA amplification from silver-stained gels Andreas Vogel
DNA analysis programs Cornelius Krasel
Blotter Cornelius Krasel
Gamma Irradiation Mr. R. Cartwright
ss-DNA-Ligation Dorian Winter
DNA analysis programs Manuel de las Calzas Largas
BioRad RotoforDIR fleming at guvax.acc.georgetown.edu
Bicinchoninic Acid (BCA) protein assay fleming at guvax.acc.georgetown.edu
image enhancement of sequencing gels Ashleigh Mckenzie
Ammonium formate Denni Schnapp
Pharmacia's EasyPrep. OKADA Yasushi
DNA Nomenclature Dr. M.D. Jones
DNA amplification from silver-stained gels Andreas Vogel
microbiology newsgroup Blumenthal
ss-DNA-Ligation Zophonias Oddur Jonsson
Quantifying minute amounts of DNA? littgj at wmvx.lvs.dupont.com
Primer Design Programs for PC Andrew Simmonds
seek antibody to HTLV-1 Marion Freistadt
Lambda RZ5 Philip Heacock
Dialog/Paperchase... Marion Freistadt
Wizard & Sequencing Roger Wiegand
DNA Nomenclature Warren Gallin
Alas, poor "magic"! I knew thee well. Warren Gallin
help with IVT STELLA TSIRKA
minipreps for sequencing txpljfg at UABCVSR.CVSR.UAB.EDU
protein purification column Hong Liu
mouse Lop-4, Graham fisher john hess
Wizard & Sequencing Brian Schmidt
antibody compendium? Jim Adams
Borate and DNA Rafa Maldonado
Polysomes run off assays Vassilia Fasoula
DNA Nomenclature David A. Adler
TAQ SEQUENCING KITS Helen McBride
Windows Mosiac experience? mhollowa at epo.som.sunysb.edu
PLEASE HELP: HOW DO YOU UNSUBSCRIBE???!! WILLARD at MVAX.WISTAR.UPENN.EDU
Dialog/Paperchase... Bob DeLisio
determining oligomeric structure of a protein Russ_Lehrman
Invitro and Invivo footprinting anjali at nii.ernet.in
parts to make automated oligo synthesizer Mike Klopfer
protein purification column Shaun D. Black
seek "Prep-a-Gene" CLEPPER at MAINE.MAINE.EDU
protein purification column Warren Wakarchuk
how to isolate a plasmid from Brevibacterium ammoniagenes? aklib at delphi.com
DNA Nomenclature Enrique Jose Labadan Frio - SCBC - 3421101
A problem in metabolic radiolabelling T. S. Pillay
Guidelines for GST fusion T. S. Pillay
ARCHIVE Stephen T Simpson
making antibodies to low antigenicity proteins Richard Cooke
Looking for GH4C1 cells K.D.Preuss
Non-Specificity for Primer Extension ? Martin Leach
SOUTHERNS Martin Leach
making antibodies to low antigenicity proteins Ding Ming
SUMMARY: no pcr products, Promega kits? Marcella E Grebus
Experience with BioRad Rotofor system newittja at delphi.com
seek "Prep-a-Gene" Manuel de Cervantes Saavedra
experiences with pET vectors from Novagen Bipin K Dalmia
DNA Electroelution Apparatus Ravi Ramachandran
DNA Nomenclature Patricia Foster
seek "Prep-a-Gene" Andrew Simmonds
E. coli flg(Hag) mutants KRUEGER, CHARLES M
minipreps for sequencing Karen L. Yoas
experiences with pET vectors from Novagen Cornelius Krasel
(none) carlos h. pedemonte
antibody compendium? carlos h. pedemonte
determining oligomeric structure of a protein David I Resnick
microbiology newsgroup Matthew Stoecker
GHOST IMAGES! MAHARAJAH
DNA Electroelution Apparatus the End
how to isolate a plasmid from Brevibacterium ammoniagenes? the End
test mbazl at seqnet.dl.ac.uk
how to isolate a plasmid from Brevibacterium ammoniagenes? wcg8744 at
Sequence of pUC120 I-shiou Ng
Windows Mosiac experience? Seth Daniel Crosby
crucifixion Seth Daniel Crosby
crucifixion Harry Menegay
making antibodies to low antigenicity proteins Roland J Saldanha
crucifixion John H McDonald
experiences with pET vectors from Novagen Anne Savitt
Mac Mosiac CC
crucifixion Michael J Marino
crucifixion littgj at wmvx.lvs.dupont.com
vector pAT153? Eric Carstens
transfection in cell suspension Therese Ann Dietlin
Mac Mosiac David S. Lee
Windows Mosiac experience? Jonathan B. Marder
parts to make automated oligo synthesizer daj (David Johnston)
Restriction enzyme berschic at convex.rz.uni-duesseldorf.de
restriction enzyme Bae I berschic at convex.rz.uni-duesseldorf.de
antibody sources copp at mcclb0.med.nyu.edu
nucleotide sequence searching via the Internet botjlh at lure.latrobe.edu.au
Ammonium formate Michael Casey
PcAb Elution problems matiasm at rand
VACCINE URGENTLY NEEDED Administracion nodo COFEMZ
Redivue radionucleotides from Amersham WMGDDM
Rapid boiling mini-prep method neale at mbcf.stjude.org
NASBA Birgitt Oeser
Avidin.Biotinlated Protein Complexes & SDS-PAGE John Altman
Agrobacterium DNA Andrea M Skantar
Kits for RNA extraction from whole blood? Karen Sebag
Mac Mosiac the End
got answers, thanks, bye john hess
methds-reagnts FAQ? Danielle Beaulieu
hydrophobic PEPTIDE Dorian Winter
Enzymes for PCR Sue Jeffrey
Degenerate PCR - Sequencing? Jim Gerlach
Vector pMT010/A+ sequence wanted Steve Eklund
Windows Mosiac experience? Dan Jacobson
Restriction enzyme Duncan Clark
Dyes for PCR Duncan Clark
formate buffer Denni Schnapp
ammonium formate Denni Schnapp
DNA amplification from silver-stained gels dkim at unm.edu
microbiology newsgroup John Nash
making antibodies to low antigenicity proteins Paul Bucciaglia
Urgent-vacine needed Terry Viancour
Apparent MW of protein on SDS-PAGE > real MW? Baohua Huang
Freezing competent E coli Richard Near
Tissue Damage Using In Situ/PCR? William Anderson
Variable yields in T7 trascritpion the End
Screening for PTHrP Vivien Bonazzi
Plasmid extraction Shahram Mori
Freezing competent E coli Rafa Maldonado
GHOST IMAGES! Mark Schweder
Apparent MW of protein on SDS-PAGE > real MW? Warren Gallin
microbiology newsgroup ed beaty
microbiology newsgroup Dan Ferber
Urgent-vacine needed David S. Lee
large primer PCR Seth Daniel Crosby
Urgent-vacine needed David S. Lee
Degenerate PCR - Sequencing? Frank Richardson
Redivue radionucleotides from Amersham Allison Hanly
unsubscribe BXCZERNIK at LIFE.UAMS.EDU
Protein A gene request Clay Oliver
bacteriocidal agent & calli brwgor01 at uctvax.uct.ac.za
Gel Shift Question gc
crucifixion Jonathan Greene
Gel Shift Question Steve Rodems
Reliable 2D electrophoresis 108KIECK at witsvma.wits.ac.za
PAGE MW estimates douglas l feinstein
chicken lysozyme Kohalmi, Susanne
bacteriocidal agent & calli Naresh C. Pancholi
What is the gt in lambda gt10 or gt11? Paul Riggs
large primer PCR the End
Biorad Protein Concentrations LYNDON at BMS-UNIT.ICR.AC.UK
microbiology newsgroup the End
"air" type thermal cyclers Marcella E Grebus
Where Can I get Hsp90 cDNA? WANGNN at UCBEH.SAN.UC.EDU
lyticase Sean David Moore
(none) WANGNN at UCBEH.SAN.UC.EDU
Chicken YAC library? dresserm at omrf.uokhsc.edu
Who is the first dicover PCR technique? Wasun Chantratita
Plasmid extraction the End
sequence of lambda gt10 Dr. A.J. Harmar
PCR discoverer shiha02 at mcclb0.med.nyu.edu
Dyes for PCR Robert Horton
Variable yields in T7 trascritpion Virginia Dress
Dyes for PCR Gerard R. Lazo
Dyes for PCR Duncan Clark
Variable yields in T7 trascritpion Edwin ten Dam
In Vivo Transfection Sue Jeffrey
differential display nobody
Thiazol (acridine) orange for PCR? IRVK at MAINE.MAINE.EDU
Dyes for PCR Duncan Clark
Freezing competent E coli Christopher Coward Path
Dyes for PCR Michael Coady
test DR. KEITH BONHAM
Test Ill-Whan Sul
erase a base hotan at
Methods for plant RNA isolation isg1930 at uxa.cso.uiuc.edu
Plasmid extraction Diana Ralston
transfection in suspension culture Therese Ann Dietlin
search for cyclosporin A txpljfg at UABCVSR.CVSR.UAB.EDU
Help with R751Helper Plasmid Molapo Qhobela
Methods for plant RNA isolation Purnima Laud
erase a base KRUEGER, CHARLES M
Redivue radionucleotides from AmershamREAD/NEXT Martin Kennedy
help method for protein estimation CRH
RDA bruced
DNA quantitation method wanted Jeff
hydrophobic PEPTIDE Dorian Winter
erase a base Rick Wilson
Methods for Plant RNA Isolation KRISTJANNSON at NCCCOT7.AGR.CA
Thiazol (acridine) orange for PCR? Parke K. Flick
PCR on phage Gustavo Glusman
In Vivo Transfection Basavaraju Shankarappa
Adding Restriction sites to Primers BMA14432 at vax1.utulsa.edu
In Vivo Transfection Jim Woodgett
request follow-up on SmaI (PCR subcloning woes cont.) txpljfg at UABCVSR.CVSR.UAB.EDU
Lac Switch Inducible Expression system wzhou at bimcore.cc.emory.edu
Degenerate PCR - Sequencing? Hank Seifert
RAPD Users ? Steve Dobson
What is the gt in lambda gt10 or gt11? Hank Seifert
Can MB be used to stain ssDNA uniquely? Jun Li
Dictyostelium HELP MUNGO at UVVM.UVIC.CA
Adding Restriction sites to Primers Warren Gallin
Gel Shift Question Song Tan
Dyes for PCR Randy P. Rasmussen
Dictyostelium HELP Virginia Dress
erase a base mhollowa at epo.som.sunysb.edu
Mac Mosiac Tracy Aquilla
calcium-phosphate transient transfections Tracy Aquilla
Mac Mosiac Tracy Aquilla
calcium-phosphate transient transfections Tracy Aquilla
DNA Nomenclature Russell A. Maurer
Dictyostelium HELP Warren Gallin
Degenerate PCR - Sequencing? Brian Foley
Adding Restriction sites to Primers David I Resnick
Alas, poor "magic"! I knew thee well. Martin Kennedy
omega fragment of Beta-galactosidase Jonathan Kurtis
PCR discoverer John Brunstein
Restriction map of pAM120 again! Wei C.
NIH Image 1.44 v.s. NCSA GelReader, which is better? Michael Man
Protein-protein interactions Steve Rodems
Adding Restriction sites to Primers choie01 at mcclb0.med.nyu.edu
Removal of RNA from minipreps ALAN J. SIMMONS
Expression in E.coli with rare codons ANDY PHILLIPS
Mink Dna Marianna Max
Anti-DNA antibody request Clay Oliver
Adding Restriction sites to Primers Brian Foley
Protein-protein interactions Song Tan
Nonradioactive vs. radiolabelled probes Wasun Chantratita
Large Scale Fermentation LYNDON at BMS-UNIT.ICR.AC.UK
ADVICE ON SSCP WANTED MED6MHS at VAX.BIO.LEEDS.AC.UK
request follow-up on SmaI (PCR subcloning woes cont.) T. S. Pillay
DNA-protein interactions Miss L.M. Fernandez
Page MW est.:response Mark Jason Logan
DNA triple helix software? NISSON at BIOTECHNET.COM
Isolating High MW DNA from Gel- Best Way?? Peter T. Boag
"air" type thermal cyclers Majed Abu-Hajir
Removal of RNA from minipreps Hanson Lab
subtraction libraries William F. Wade
in situ with short probes JIA at CUCCFA.CCC.COLUMBIA.EDU
ribosomal binding site Bulletin board login
Need HSP60 probe Michael Delgado
omega fragment of Beta-galactosidase Michael Benedik
Lac Switch Inducible Expression system Hong Dang
Biorad Protein Concentrations OPUS
disrupting e. coli cells Bipin K Dalmia
Freezing PCR products Marion Freistadt
Alas, poor "magic"! I knew thee well. helgew at LJCRF.EDU
Advantages of 33-P jost at vaxc.cc.monash.edu.au
minute amounts of DNA/cuvettes rhubner at molbiol.ox.ac.uk
disrupting e. coli cells Ben Davis
Blocking of repeats for cosmid hybridization Ola Myklebost
Freezing PCR products bss032 at vaxa.bangor.ac.uk
DNA-protein interactions Song Tan
Insta Preps Reference Allison Hanly
ADVICE ON SSCP WANTED M K Bennett
Source for ISOQUICK? Ian Frame
Source for ISOQUICK? Ian Frame
Adding Restriction sites to Primers Dr. Robert Darnell
disrupting e. coli cells Basavaraju Shankarappa
Advantages of 33-P Dan Diaz
Proofreading x engineering terminal sites Petr GRUZ
Freezing PCR products Jim Owens
Advantages of 33-P John Nash
minute amounts of DNA/cuvettes John Nash
PCR on phage Gustavo Glusman
Insta Preps Reference John Nash
DNA from Gel - GeneClean Tony Hodge
cloning from agarose brett at BORCIM.WUSTL.EDU
Kits for RNA extraction from whole blood? Menoud Pierre-Alain
Protease Deficient E. coli for GST fusions LICHY at EMAIL.AFIP.OSD.MIL
DNA from Gel - GeneClean Andrew Simmonds
Discovery of PCR principle Olav Hungnes
DNA Nomenclature Paul Riggs
disrupting e. coli cells Michael van Waes
Coomassie staining Paul Riggs
Have you used the DNA dye (SYBR GREEN I) from Molecular Probes? carlos h. pedemonte
TEST Disregard JOHNSON_T
Non-porous SPE Phases Carl Weatherell
ribosomal binding site a Could someone Bonham, Peta
Protease Deficient E. coli for GST fusions Anne Savitt
erase a base Andrew Tai
cloning from agarose Cornelius Krasel
plastic disposable columns donald seto
PCR cloning - a protocol txpljfg at UABCVSR.CVSR.UAB.EDU
DNA Nomenclature Russell A. Maurer
need HELP with non-radioactive Northern hybs Tracy Aquilla
Coomassie staining Ding Ming
test Preecha Leangaramgul - SCBT - 3636197
RNA extraction of fungi Preecha Leangaramgul - SCBT - 3636197
What is the gt in lambda gt10 or gt11? Brian Smith-White
omega fragment of Beta-galactosidase Brian Smith-White
need HELP with non-radioactive Northern hybs John Nash
cloning from agarose Richard Near
DNA analysis programs Greg Denomme
Freezing Antibodies... Andrew Simmonds
PCR on phage Allison Hanly
Insta Preps Reference Allison Hanly
Butanol purification of phosphorothioates Philip L. Carl
(Dis) Advantages of 33-P Dan Diaz
Nuclease Inhibitors Scott Selby Med Heme
"quantitative"? paul haney
Butanol precipitation of phosphorothioate oligonucleotides Philip L. Carl
disrupting e. coli cells Pete Dunten
Protease Deficient E. coli for GST fusions Bipin K Dalmia
Cell surface Biotinylation Peter Smith
Diluted ECL Reagent ? George W Chacko
APOE Pcr Gregory P. Riddick
plastic disposable columns Allison Hanly
Freezing competent E coli Bernard Heymann
Cot curve for Zea Mays? Nigel Walker
Comments on Locus pocus-Novagen Matthew Wakefield
peptide antibodies Peggy Kolm
Alas, poor "magic"! I knew thee well. Martin Kennedy
PCR on phage Martin Kennedy
What is the gt in lambda gt10 or gt11? Mark D. Garfinkel
sequenasePCR Margaret M Lanterman
Degenerate PCR - Sequencing? Margaret M Lanterman
Diluted ECL Reagent ? Margaret M Lanterman
lambda nm1149 Tracey Zacharias
Removal of RNA from minipreps Warren Voth
Pseudomonas LPS Callicot at utkvx.utk.edu
DNA analysis programs Klaus.Matthaei at ANU.EDU.AU
NASBA Birgitt Oeser
Proofreading x engineering terminal sites Brian Foley
Cleaning of sequencing gel plate wzhou at emoryu1.cc.emory.edu
plastic disposable columns Song Tan
Designing IPCR primers Emily Lawson
ANGIOTENSINOGEN ASSAY MED6MHS at VAX.BIO.LEEDS.AC.UK
Degenerate PCR Seth Findley
Cleaning of sequencing gel plate Jim Owens
Advantages of 33-P Jim Owens
Cleaning of sequencing gel plate Zophonias Oddur Jonsson
Expression in E.coli with rare codons mccallo at rand
What is the gt in lambda gt10 or gt11? Hank Seifert
Degenerate PCR Warren Gallin
Cell surface Biotinylation Anita Gould
Salmon vs. Herring Testes Randal W Giroux
Degenerate PCR John Nash
Heteroduplex Analysis Song-Muh Jong
PcAb elution problems JOHN PROUDMAN
Silver staining with ampholytes JOHN PROUDMAN
methylases? Karl Voss
plasmid integrated or not ? AT
erase a base Tanya Osickey
problem thermal cyclers (techne> Grant McKegney
Cleaning of sequencing gel plate T. S. Pillay
Diluted ECL Reagent ? Ron Wange
Diluted ECL Reagent ? Ron Wange
Advantages of 33-P Dan Diaz
crucifixion Shiao Y. Wang
PCR then Sequence Shiao Y. Wang
P. falciparum cDNA lib? Fourie Joubert
plasmid integrated or not ? Jarmo Niemi
estrogen and pheromones Dion Murphy
Dictyostelium HELP micprf at lure.latrobe.edu.au
Have you used the DNA dye (SYBR GREEN I) from Molecular Probes? Hanne Skomedal
plastic disposable columns Allison Hanly
Have someone done Inverse-PCR on integrated virus? Hanne Skomedal
Search for a TA3Ha (expression) library hvos at nki.nl
(none) sjjdlli
Electroporation of Mammalian cells Mr. J.H. Slingsby
lambda nm1149 Mark D. Garfinkel
Southern blot problem lamoreauxw at msuvx1.memst.edu
Freezing Antibodies... CC
lignin isolation jodi norris
Alas, poor "magic"! I knew thee well. Cornelius Krasel
PCR review? ed beaty
Freezing Antibodies... Virginia Dress
Silver staining with ampholytes Virginia Dress
dsDNA site mutations JOHNSON_T
RNA/sucrose density Schu Bernhard
Apparent MW of protein on SDS-PAGE > real MW? Eric Carstens
Autoradiograph Binders?? SCOTTA at NCCCOT7.AGR.CA
PCR difference cloning William F. Wade
New Phage ssDNA preparation kit <-- well not really a kit ;-) John Nash
dsDNA site mutations Tony Sanchez
disrupting e. coli cells David LaPorte Biochem
About Pharmacia...... Ding Ming
DNA analysis programs Bob Gross
ssDNA preparation Rick Wilson
Alas, poor "magic"! I knew thee well. Warren Gallin
PCR on phage Nicola Collie
use of Pfu versus Taq: conditions? rdkortschak at science.adelaide.edu.au
What is the gt in lambda gt10 or gt11? Gregory Harriman
DNA from Gel - GeneClean Mark Jason Logan
quick pcr Daniel Petersen
Haemophilus influenzae plasmids Patricia.Moor at ANU.EDU.AU
Autoradiograph Binders?? daj (David Johnston)
Was: About Pharmacia...... about the cost of equipment manuals daj (David Johnston)
RNA extraction of fungi Preecha Leangaramgul - SCBT - 3636197
differential expression gene Preecha Leangaramgul - SCBT - 3636197
Blunt end Ligation anjali at nii.ernet.in
Loss of plasmid in liquid culture RROHAN at UMAB.BITNET
lambda nm1149 futers at bpxtal.leeds.ac.uk
Blobs on 35S sequencing gels Dr. M. Rhodes
Biometra Tony Sanchez
phage display library mlange at nki.nl
Help needed for expression in 293 cells Ashu Sharma
The sizes of biotin, digoxigenin, FITC, etc. Wasun Chantratita
intensifying screens Steven Hecht
lambda nm1149 Mark D. Garfinkel
plastic disposable columns tan at aeolus.vmsmail.ethz.ch
Alas, poor "magic"! I knew thee well. Roger Wiegand
Double stranded DNA sequencing gc
New Phage ssDNA preparation kit <-- well not really a kit ;-) the End
Biometra John Brunstein
Loss of plasmid in liquid culture Anne Savitt
quick pcr Daniel Petersen
Kan-inducible promoter Y.C.
Long PCR Wayne M. Barnes, Ph.D.
Kan-inducible promoter Y.C.
Carborundum and transfer of high MW molecules kirtikara at aesop.rutgers.edu
Sequencing with deaza-dGTP and signal loss Bill Nowatzke
Double stranded DNA sequencing Susan L Forsburg
Opinions on PCR machines Susan L Forsburg
In need of Thioredoxin reductase Avi Danon
Human cDNA Lambda Zap library Rick Davis
Plasmid extraction Julie Forney Menin
Advantages of 33-P Jim Owens
2D gel HELP ! mary beard
quick pcr (colony screening by PCR) FrohlichM
What happens to 32P-dCTP when it goes KABLOOEY? wolffie at u.washington.edu
peptide antibodies Shaun D. Black
Haemophilus influenzae plasmids Patricia.Moor at ANU.EDU.AU
Loss of plasmid in liquid culture Rafa Maldonado
gel documentation/image analyis Grant McKegney
Blunt end Ligation Rafa Maldonado
Densitometry software Leonard Lutter
freezing temperature of TE Patricia.Moor at ANU.EDU.AU
GOLD LABELLING OF OLIGOS dpp at icbr.ifas.ufl.edu
ssDNA preparation Shiao Y. Wang
Advantages of 33-P jost at vaxc.cc.monash.edu.au
{DNA isolation from plant cell ez008413 at dale.ucdavis.edu
Advantages of 33-P Song Tan
pGEX-4T Expression Vector Problems Tony Hodge
Isolating High MW DNA from Gel- Best Way?? Tony Hodge
In-situ RNA-PCR Hanne Skomedal
Bis tris propane ? Joel Caillet
2D gel HELP ! Siggi Engelbrecht
Help! c-Yes antibody Reto Hartmann
gel documentation/image analyis Hank Seifert
The sizes of biotin, digoxigenin, FITC, etc. Wasun Chantratita
Chelex 100 question Peter Thorén
Chelex 100 question, ignore previous posting Peter Thorén
post pesusi at sara.cc.utu.fi
Isolating High MW DNA from Gel- Best Way?? the End
quick pcr (colony screening by PCR) Emanuele Buratti
Opinions on PCR machines the End
G418 in plasmid prep Scott Selby Med Heme
Cosmid & Lambda Sequencing on the ABI 373A. Alan Lyons
Lithium Isethionate source Gary Rudnick
Cell surface Biotinylation Gary Rudnick
Coli strain for periplasmic expression Deborah Hodges Rheumatol
pGEX-4T Expression Vector Problems Mark Jason Logan
Opinions on PCR machines Parke K. Flick
TA cloning KRUEGER, CHARLES M
densitometry software 4700gbera at UMBSKY.CC.UMB.EDU
What is the gt in lambda gt10 or gt11? Brian Smith-White
Sequencing gel apparatus suggestions Brian T. Greuel
Loss of plasmid in liquid culture Michael Benedik
Coli strain for periplasmic expression Michael Benedik
Non-radioactive Labelling Kits IXK3 at psuvm.psu.edu
plasmid integrated or not ? Brian Smith-White
Oligo size for microinjection? Don Grieco
Carborundum and transfer of high MW molecules Helen McBride
quick pcr john hess
Isolating High MW DNA from Gel- Best Way?? Alejandro Abuin
unsubscribe sreddy at OVCNET.UOGUELPH.CA
need HELP, blah blah Paul N Hengen
Freezing PCR products Paul N Hengen
Freezing PCR products Paul N Hengen
DNA/Oligos delivery into the tough guys - RBL2H3's Michael J. Tuvin
About Pharmacia...... Virginia Dress
Advantages of 33-P Brian Smith-White
quick pcr (colony screening by PCR) Dave Dudley
need HELP, blah blah Helge Weissig
Influenza HA tag batu erman
Blunt end Ligation Marinella Callow
Non-radioactive labelling kits JOHN PROUDMAN
What happens to 32P-dCTP when it goes KABLOOEY? Brian Smith-White
About Pharmacia..... JOHN PROUDMAN
n Nigel Appleton
hydrofobicity Mauricio Cesar Palmieri
The "RACE" for 5'ends Matthias Zeiner
What is the gt in lambda gt10 or gt11? Mark D. Garfinkel
Freezing PCR products Jim Owens
Carborundum and transfer of high MW molecules Andrew Cockburn
Poor capillary transfer? douglas l feinstein
Info on French Company Ozyme JEMERTON at BIOTECHNET.COM
Large-scale preparations of plasmid DNA, what wrong? Huanquan Zheng
[Q} Matchmaker Two hybrid system, anyone using it? Zhongguo Xiong
The "Race for 5'ends Matthias Zeiner
Poor capillary transfer? Tony Sanchez
test rep at icbr.ifas.ufl.edu
Info on French Company Ozyme Manuel G. CLAROS
Lithium Isethionate source Pierre Jelenc
Autoradiograph Binders?? Andrew Cockburn
Ancient messages reappearing...... John Brunstein
G418 in plasmid prep Hanson Lab
Poor capillary transfer? Hanson Lab
MSDS Earnest Borgnine
About Pharmacia...... Virginia Dress
In situ PCR references Linn, Charles Ernest
RNA extraction of fungi Marcello.Marchiani at bota.unine.ch
MSDS Helge Weissig
Biometra Steve Minchin
MSDS John Brunstein
About Pharmacia...... Helge Weissig
lac-Z with CMV promoter vector? John Storring
Diluted ECL Reagent ?DIR fleming at guvax.acc.georgetown.edu
lambda vector map- charon 4 Dr. Chihara
About Pharmacia..... Ding Ming
FLAG expression vector source? ed beaty
use of Pfu versus Taq: conditions? Michael Benedik
How low can you go (Nuc Acid pellets)? David Sang-shin Lee
quick pcr (colony screening by PCR) john hess
PCR primers for chromosome markers - sources wanted Mark J. Miller
Screening phage lib. by PCR? Paul Edward Kowalski
About Pharmacia...... Helge Weissig
What is the gt in lambda gt10 or gt11? Brian Smith-White
[Q} Matchmaker Two hybrid system, anyone using it? Seth Corey
Large-scale preparations of plasmid DNA, what wrong? Brian Smith-White
Anti-phosphoserine monoclonals? Seth Corey
Large-scale preparations of plasmid DNA, what wrong? Thomas H. Rude
"air" type thermal cyclers Shiao Y. Wang
sequenasePCR Alistair Forrest
Sequencing PCR products Shiao Y. Wang
{DNA isolation from plant cell Peter Gegenheimer
Degradation time of 3' A overhang in TA cloning J. David Spafford
Blunt end Ligation Martin Kennedy
Loss of plasmid in liquid culture Martin Kennedy
Alas, poor "magic"! I knew thee well. Martin Kennedy
G418 in plasmid prep Martin Kennedy
HELP - Taq versus Pfu jsecombe at science.adelaide.edu.au
Influenza HA tag Vuk Stambolic
How low can you go (Nuc Acid pellets)? James B. Hutchins
How low can you go (Nuc Acid pellets)? Robert Roxby
thermocycler summary Susan L Forsburg
On-line Chemical Catalogs? Chris Yang
On-line Chemical Catalogs? Andrew Simmonds
Expedite chemistry oligo synthesis James Gibbs
IMPORTANT BIOSCI INFORMATION David Kristofferson
Promoter for Ampicillin in plasmids. h9290184 at hkucc.hku.hk
Re. : promoter for Ampicillin in plasmids GWDGV1::KCHOWDH
LOST PLASMID.....!(can never be found) MAHARAJAH
Loss of plasmid in liquid culture Duncan Clark
RNA extraction of fungi Cornelius Krasel
IMPORTANT BIOSCI INFORMATION 3QY at QUCDN.QueensU.CA
Anti-phosphoserine monoclonals? Cornelius Krasel
Multiplex PCR design program Jose_Costoya
FLAG expression vector source? Eric Carstens
Influenza HA tag Preston Garrison
Apurinic sites? marodriguez
APOE Pcr Timo Hiltunen
digital cameras E.L. Newman Surgery ext ninewells 3174
how low can you go (nucl. acids pellets)? suter at VAX.MPIZ-KOELN.MPG.d400.de
About Pharmacia...... Nigel Appleton
biomoo neuroscience journal club David Mckalip
TA cloning Brian Foley
Sequencing gel apparatus suggestions Sean David Moore
IMPORTANT BIOSCI INFORMATION Dean Danner Lab
Re-use of electroporation cuvettes? Mr R D Williams
Salmon vs. Herring Testes Stanley Tahara
looking for rpsL plasmid Szatmari George
Re-use of electroporation cuvettes? John Gosink
ATCC on the net somewhere? Helmut Dotzlaw
Anti-phosphoserine monoclonals? Anne Savitt
(none) Fernley Symons
Expression of fusion proteins Alan H. Schulman
Knockout mice Michael Coady
plasmid integrated or not ? AT
Re-use of electroporation cuvettes? Harry Menegay
How low can you go (Nuc Acid pellets)? Brian Foley
Non-radioactive Labelling Kits Casey Finnerty
lambda vector map- charon 4 Dr. Chihara
Charon 4 and/or Charon 4A ??? Dr. Chihara
Asking for Vero cells Hinayana Bawagan
sequencing 60 bp? Tracey Zacharias
Adding linkers to PCR products Julie Wilberding
ATCC on the net somewhere? Helge Weissig
sequencing 60 bp? Martin Leach
FUNGAL RNA EXTRACTION Philip OBrien
Deletions by site directed mutagenesis ZURLINDEN at IMB.unibe.ch
Re-use of electroporation cuvettes? Manuel G. CLAROS
G418 (for selection) Dr M Fahmy
post Tony Sanchez
dsDNA site mutations Sue Jeffrey
Poor capillary transfer? Wasun Chantratita
Re-use of electroporation cuvettes? Przemko
Deletions by site directed mutagenesis BSS032 at BANGOR.AC.UK
The "RACE" for 5'ends ajwatson
About Pharmacia..... Shiao Y. Wang
transfecting COS cells Val Snewin
cDNA Library Elaine Bly
Request for Thermolyne Thermocycler recommendations DAVID F. BISHOP
Advantages of 33-P Parent Stephane
looking for e-mail address of Drs. Francois Deryckere and Frank Gannon Tao Wei
Plant cDNA Library J. Zwiazek
Matchmaker 2 hybrid system Carol Scaramuzzi
High Volume Chemistry Analysers Samuel D. Hunt
G418 Graham Atherton
Blunt end Ligation micprf at lure.latrobe.edu.au
A375 cDNA library available??? hvos at nki.nl
Cheap substitute for Dynabeads? Helgi Briem Magnusson
Salmon vs. Herring Testes Mike Morris
Inverse PCR Matthias Zeiner
need source for Xca I restriction endonuclease Tracy Aquilla
G418 Hanson Lab
Deletions by site directed mutagenesis Parke K. Flick
About Pharmacia...... Duncan Clark
glued gels sticking to both plates Eric Kofoid
Cheap substitute for Dynabeads? Helgi Briem Magnusson
(none) sjjdlli
Diatom. Earth ref. Bruce E. Taillon
Loss of plasmid in liquid culture Paul N Hengen
Cheap substitute for Dynabeads? Paul N Hengen
the "Eijen" <sic> for fluor-PCR? John Alsobrook
Advantages of 33-P John Alsobrook
FAQlist for bionet.molbio.methds-reagnts Paul N Hengen
Anchored Primer protocol? russos at HAL.HAHNEMANN.EDU
Human cDNA Lambda Zap library Sarven
looking for a mice heart cell line Michael Man
mycophenolic acid,aminopterin, MAX may
adenylate cyclase defficient cells carlos h. pedemonte
(none) krishnan at BORCIM.WUSTL.EDU
the "Eijen" <sic> for fluor-PCR? Paul N Hengen
HELP - Taq versus Pfu NEAL MUSTO
RNA from CsCl or Quiagen plasmid? JOHNSON_T
Ghost plasmid of pBluescript Paul N Hengen
Capillary electrophoresis of proteins Paul N Hengen
Qiacrap --> RNA brett at BORCIM.WUSTL.EDU
Translation initiation in drosophila Nico Stuurman
Advantages of 33-P Dan Diaz
RNA from CsCl or Quiagen plasmid? Andrew Simmonds
DNA Extraction from methanol preserved gastropods Andrew McArthur
problem in 2D-PFGE David Chiu
Diatom. Earth ref. David Chiu
Re-use of electroporation cuvettes? malaine at sara.cc.utu.fi
Problems with synapsys jreich at illite.u-strasbg.fr
Adding Restriction sites to Primers micprf at lure.latrobe.edu.au
need source for Xca I restriction endonuclease Pierre Martineau
Enzyme test for ghost bands bss032 at vaxa.bangor.ac.uk
quick pcr (colony screening by PCR) Chong-Yee Khoo
Adenylate cyclase defficient cells carlos h. pedemonte
Apurinic sites? Brian Smith-White
Re-use of electroporation cuvettes? Peter M. Muriana
Diatom. Earth ref. Paul N Hengen
quick pcr (am arrivals) Dean Danner Lab
Prokaryotic ribosomal gene wanted WILLIS at BCRSSU.AGR.CA
digital cameras Torsten Klade
Enzyme test for ghost bands Paul N Hengen
(none) ajwatson
obtaining DNA from live mice Mary Lamb
Homemade nonrad. blot detection Richard Near
quick pcr (colony screening by PCR) Bernard Murray
test wzhao at mbcr.bcm.tmc.edu
test wzhao at mbcr.bcm.tmc.edu
Human Tumor Southerns Fred Boyd, Ph.D.
XL1Blue MRF' wzhao at mbcr.bcm.tmc.edu
XL1Blue MRF' Michael Coady
PCR for 6-7 kb Nina.Schweigert
How low can you go (Nuc Acid pellets)? Jim Owens
Equipments Janis Eisaks
FDA database Shaojiu Wang
Serum rich medium for lymphocyte cultures- any recipes? Kurt Schaudt
help needed on gel retardation assay gc
Degradation time of 3' A overhang in TA cloning Andrew Harrison
Ghost bands wanted for enzyme test bss032 at vaxa.bangor.ac.uk
PCR for 6-7 kb Lukasz Salwinski
GeneAmp RNA PCR kit questions J. Logan Black
Polyoma virus BMA14432 at vax1.utulsa.edu
Bio-Rad Prep-A-Gene wzhao at mbcr.bcm.tmc.edu
looking for a mice heart cell line Michael Man
disrupting e. coli cells Bill Jack
** DEPC and reagents ** Helge Weissig
Bio-Rad Prep-A-Gene James S. Sutcliffe
Promoter for Ampicillin in plasmids. Bill Jack
Northern Problem KGEISS%RAVEN at EAGLE.AAMRL.WPAFB.AF.MIL
Bio-Rad Prep-A-Gene Dr. Chihara
Bio-Rad Prep-A-Gene Dr. Chihara
** DEPC and reagents ** Ding Ming
blunt-ending PCR product mcgraw at BSCR.UGA.EDU
weird microsatellite mcgraw at BSCR.UGA.EDU
help needed on gel retardation assay Tao Wei
looking for a mice heart cell line Marion Freistadt
blunt-ending PCR product john hess
mRNA differential display. Bjorn Larus Orvar
AG BIOTECH INFO via INTERNET! Daniel M. Cabirac
PCR for 6-7 kb Ashok Aiyar
PCR for 6-7 kb Dan Diaz
ATCC on the net somewhere? Lynda Callicotte
Loss of plasmid in liquid culture Duncan Clark
Bio-Rad Prep-A-Gene Patricia Foster
Sequencing gel haze near bottom Ronald Meis
Promoter for Ampicillin in plasmids. John Brunstein
Transposon mediated deletion, Gibco Deletion fasctory wzhou at emoryu1.cc.emory.edu
Adding Restriction sites to Primers Anita Gould
RNA Gels... Andrew Simmonds
Northern Problem MALBOOBI at QUCDN.QueensU.CA
help needed on gel retardation assay Martin Leach
adenylate cyclase defficient cells Steven Coon
RNA from CsCl or Quiagen plasmid? Steven Coon
glued gels sticking to both plates Steven Coon
Northern Problem Steven Coon
Double stranded DNA sequencing Stephen R. Lasky
test - Ignore Anthony Hilton
Adding Restriction sites to Primers Christopher Cilley
(none) Milton Taylor
mRNA extraction kit Y.R. Boisclair
Double stranded DNA sequencing Charles J. O'Kelly
Amplifying with glutamine synthase John Harpel
PCR >6 kb brett at BORCIM.WUSTL.EDU
weird microsatellite Martin Kennedy
mRNA from Total RNA saast8 at vms.cis.pitt.edu
baculovirus and PCR Shiao Y. Wang
baculovirus and PCR Steve Rodems
Which pipetters to purchase? J. David Spafford
SV-40 promoter L.Y.Chau
custom antibodies Matthias Zeiner
primer design for PCR suter at VAX.MPIZ-KOELN.MPG.d400.de
Which pipetters to purchase? Torsten Klade
Which pipetters to purchase? malaine at sara.cc.utu.fi
Fluorescent sequencing of high MW dsDNA krishnan at BORCIM.WUSTL.EDU
Double stranded DNA sequencing Stephen R. Lasky
RNA Gels... Hanson Lab
Protease Deficient E. coli for GST fusions Paul Riggs
need source for Xca I restriction endonuclease Patty McManus
How to dry a polyacrylamide sequencing gel on the glass plate. Wasun Chantratita
ATCC on the net somewhere? Chaisiri Wongkham
Matchmaker 2 hybrid system Zhongguo Xiong
Lobster or shrimp cell lines Wasun Chantratita
The sizes of biotin, digoxigenin, FITC, etc. Wasun Chantratita
Blobs on 35S sequencing gels Zophonias Oddur Jonsson
histone/rRNA primers APARKER at MAINE.MAINE.EDU
Which pipetters to purchase? John H McDonald
LambdazapII joyce setsuda
mice heart cultured lines Marion Freistadt
How low can you go (Nuc Acid pellets)? Marion Freistadt
Serum rich medium for lymphocyte cultures- any recipes? Marion Freistadt
summary: Degradation time of 3' A overhang J. David Spafford
DNA isolation from blood ww40
probe cleaning B Stickney
cDNA libraries Jean-Pierre Paccaud
SynapSys Phone Number? jeff
pOX38 Condie Carmack
3H-T labelling in cell culture mei deng
sequence of BRL pAMP-1? Richard R. Hardy
PCR based site-specific Mutagenisis Simon Twigger
Reverse Transcriptase Question Life Technologies Tech-Line
How to dry a polyacrylamide sequencing gel on the glass plate. Joyce Bock
About Pharmacia............... rhubner at molbiol.ox.ac.uk
Sequencing gel combs (leakage) the End
Subscription k.reed at MAILBOX.UQ.OZ.AU
mRNA purification kit Nathalie Landry
mRNA extraction kit Nathalie Landry
Glycogen v tRNA and OLD BONES Scrimshaw, Brian J
RNA Gels... Klaus.Matthaei at ANU.EDU.AU
Sequencing gel combs (leakage) Helge Weissig
DNA from Gel - GeneClean Bob Rutledge
PCR >6 kb Alistair Forrest
Reverse Transcriptase Question Warren Gallin
Which pipetters to purchase? Shiao Y. Wang
Re. Diff.display mRNA borvar@unixg.ubc.ca babalola at MAIL.SAS.UPENN.EDU
Double stranded DNA sequencing Martin Leach
SV-40 promoter Martin Leach
Blobs on 35S sequencing gels Martin Leach
Sequencing gel combs (leakage) Brian Foley
Lobster or shrimp cell lines Martin Leach
FAQs-n-stuff! Re-post. Brian Foley
Matchmaker 2 hybrid system CLONTECH at BIOTECHNET.COM
baculovirus and PCR Allison Hanly
RNA from CsCl or Quiagen plasmid? Allison Hanly
probe cleaning futers at bpxtal.leeds.ac.uk
Gold Biotechnology and X-Gluc E. Hack
sequence schreiter at biologie.uni-halle.d400.de
shrimp cell cultures Denni Schnapp
new BuildSampleSheet for ABI sequencer Jaime Prilusky
triton-X 1OO in sequencing reaction?? URA1335 at ARTHUR.CITI2.FR
Fluorescent sequencing of high(er) MW dsDNA krishnan at BORCIM.WUSTL.EDU
Sequencing gel combs (leakage) Jim Owens
Sep Pak source CAUER at canr1.cag.uconn.edu
Sequencing gel combs (leakage) Michael G. Tencza
Phage display library Richard B. Taylor
bench, cooled microcentrifuges dpetersn at unixg.ubc.ca
Sequencing gel combs (leakage) the End
RNA probe columns NEWLIN_C
repost Triton-X boiling ss prep ? the End
DNA isolation from blood Hildegard Haas
Cleaning seq. plates with "Bon Ami"?? Steven Balt
Co-transformation of E. coli? Patricia Foster
Sequencing gel combs (leakage) Warren Gallin
sequence of BRL pAMP-1? Michael Coady
KlenTaqLA brett at BORCIM.WUSTL.EDU
PCR from lambda library Martyn White
Stratagene's pBK-CMV? bates
DNAX e-mail address? neale at mbcf.stjude.org
Sequencing gel combs (leakage) Ludwig Lab-UCSC
probe cleaning Klaus.Matthaei at ANU.EDU.AU
5' biotin labeling of oligos Liam E Good
Sep Pak source Prasad, Krishna
PCR from lambda library Warren Gallin
mRNA purification kit Warren Gallin
Sequencing gel combs (leakage) Michael Coady
PCR-sequencing schoenbaechl at urz.unibas.ch
Do you know where I can find E. coli GC6?? Christine Ward
Sequencing gel combs (leakage) Christopher Cilley
Jr. High student looking for career info. Ed Schutz
Cleaning seq. plates with "Bon Ami"?? Dan Diaz
KlenTaqLA Roberto Albinoni
Sequencing gel combs (leakage) Jarmo Niemi
How to dry a polyacrylamide sequencing gel on the glass plate. Mark
Using DNA probes to screen expression libraries HRMBDKC at HKUMD1.HKU.HK
probe: incorporation measurement Klaus.Matthaei at ANU.EDU.AU
KlenTaqLA Klaus.Matthaei at ANU.EDU.AU
Protease Inhibitors schren13 at uctvax.uct.ac.za
screening cDNA expression libraries HRMBDKC at HKUMD1.HKU.HK
Gold Biotechnology and X-Gluc E. Hack
reverse transcriptase question Dr. C. Dolphin
KlenTaqLA Ashok Aiyar
test MACHADO
5' biotin labeling of oligos Parke K. Flick
FDA database Shaojiu Wang
pUC118 / 9 MACHADO
Gamma counter Seth Daniel Crosby
Electroporation of Mammalian cells Dean Danner Lab
Cosmid Sequencing Martin Leach
Double stranded DNA sequencing Peter Gegenheimer
Glycogen v tRNA and OLD BONES Peter Gegenheimer
Re. : promoter for Ampicillin in plasmids Christopher Noren
Non-Radioactive Sequencing (Manual) G K L Munro
RNA protection assays Richard H. Smoke
(none) Marion Freistadt
RT and biotinylated RNA Joe Crouse
C. elegans DNA Steve Carlisle
Sequencing gel haze near bottom Ron Kagan
GELase or GELace MARC J GLUCKSMAN- STRUCTURAL NEUROBIOLOGY LAB
EMAIL address for Dr. L. Pegoraro ALLEN, BEVERLY S
Topoisomerase I Joe Crouse
Glycogen v tRNA and OLD BONES Uttam Rajbhandary
Sequence of pASK60/strep? Annette Meyer
Chickens? U55083 at uicvm.uic.edu
GELase or GELace John Brunstein
Bio-Rad Prep-A-Gene Wayne Woods
RNA Gels... Martin Kennedy
LOST PLASMID.....!(can never be found) Martin Kennedy
Software for sequence alignment? Paul Davis
PCR based site-specific Mutagenisis Andy
C. elegans DNA Ron Kagan
Chickens? Martin Leach
search for strain collections Dmitry A. Los
GELase or GELace malaine at sara.cc.utu.fi
RNA Gels-compendium Andrew Simmonds
Test, please ignore DARREN R TYSON
Magic - Wizard Mr V. Schoenfeld DOM
HELP Jurijs Mahinovs
long range PCR!!! Seeking papers!!! russos at HAL.HAHNEMANN.EDU
Apoptosis kit available? Katrin Adlkofer
Prof CC Wang's Address sought Ronald Berry
Non-Radioactive Sequencing (Manual) Wasun Chantratita
PCR-sequencing on colonies Svend Kjaer
"replacing" SDS with Triton X100 Walt Lilly
<None> polk at molbiol.ox.ac.uk
adenylate cyclase defficient cells Nantel Francois
RNA Gel polk at molbiol.ox.ac.uk
non-vertical bands on agaorse gels... rhubner at molbiol.ox.ac.uk
LexA or hemaglutinin Antibodies?? Seth Findley
pUC118 / 9 Francis Ouellette
Drying of PAGE gels - Cellophane Jens Alfken
e-mail address? jac33 at smhms.imperial.ac.uk
SUMMARY Sequencing gel combs (leakage) the End
CHEF-vs-ROFE Tony Sanchez
Long PCR for Australians Wayne M. Barnes, Ph.D.
Clonemaster 96-well replicator - phone #? Russell K. Chan
looking for ppGpp John Brunstein
e-mail address? Francis Ouellette
PCR-sequencing on colonies ajwatson
Stratagene's pBK-CMV? Marianna Max
DOPAMINE RECEPTOR IHC Irving Nadelhaft
PCR for 6-7 kb Jeffrey Vance
Drying of PAGE gels - Cellophane Virginia Dress
Cleaning seq. plates with "Bon Ami"?? Jeffrey Vance
PCR-sequencing Jeffrey Vance
Kanamycin or G418 for plant transformation? Jason Lei

Last message date: Thu Mar 31 23:01:54 EST 1994
Archived on: Sun Mar 6 20:03:45 EST 2005

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