proteins and plasma clearance

Martin Leach leach at
Tue May 3 22:31:18 EST 1994

In article <2q5deq$5iq at>, Hiesberger Thomas
<hiesi at> wrote:

> >
> Hello
> In my experiments I injected different proteins into the veins of
> >animals. But after the injections I discovered that the proteins were
> >degraded very quickly (60 % were not precipitable with TCA after 15
> >min.). 
> >
> >Is anybody able to tell me which part of the immune system is responsible
> >for this? Has anybody done similar experiments, or can anyone provide me
> >with a literature reference? Perhaps somebody can give me a hint as to
> >how I can inhibit or decrease this very fast degradation.

Which veins are u injecting into....

My pharmacokinetics are pretty rusty (should know this stuff as i am in a
pharmacology dept.) How about the liver. May be first pass effect.
(hmmm.... not sure if this is for veins as well as arteries.)

Could also be the blood cells (WBC's) taking up and destroying this foreign
body. T-cells??

Martin 'feeling ashamed as he knows little pharmacokinetics - professor
will be ragging on him now forever'  Leach


.....          Martin Leach                Email:leach at 
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329         
 _/  |-/__==/  80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118            "Not the old underpants on your
               USA                           head.....WIBBLE" -BLACKADDER 
p.s. try BioMOO (virtual biology on the internet - telnet 8888)

More information about the Methods mailing list