A readable but not aesthetic sequencing gel
bawagan at umdnj.edu
Tue May 3 00:47:19 EST 1994
My sequencing gels can give me the information I want but they are not
beautiful. I just hate this. I get lanes that tend to constrict. I do blow
the airbubbles off the wells to remove the urea with a glass pipette. As
soon as the temp is around 50, I load the samples but not fast enough to keep
the temp from dipping to below 45. I have trouble reusing the same tip when
others in my lab can just use one and seldom get it clogged.
I am using the Sequenase Version 2 kit. Anyone out there can give me
their reliable bag of tricks that you can not get from a book but can only
be learned from experience.
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