Heat denaturation of Sequencing
bl275 at cleveland.Freenet.Edu
Wed May 4 01:11:33 EST 1994
In a previous article, wzhao at mcbr.bcm.tmc.edu () says:
>I am looking for a protocol of sequencing double-stranded DNA
>by heat denaturation. Or any other quick double-stranded DNA
>Thanks in advance.
i've had the best success using USB's quick denaturation plasmid sequencing
kit. you add NaOH to your plasmid-primer mixture and incubate 10 min at
37 'C. then add HCl, reaction buffer and anneal and go.
More information about the Methods