Heat denaturation of Sequencing

Dan Diaz bl275 at cleveland.Freenet.Edu
Wed May 4 01:11:33 EST 1994


In a previous article, wzhao at mcbr.bcm.tmc.edu () says:

>Hi, there:
>
>I am looking for a protocol of sequencing double-stranded DNA 
>by heat denaturation. Or any other quick double-stranded DNA
>sequencing protocol.
>Thanks in advance.
>
>Wei
>

i've had the best success using USB's quick denaturation plasmid sequencing
kit.  you add NaOH to your plasmid-primer mixture and incubate 10 min at
37 'C.  then add HCl, reaction buffer and anneal and go.



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