EthidiumBr+ABI 373a??

Robert Preston rapr at MED.PITT.EDU
Wed May 4 16:35:21 EST 1994


Netfolks: has anyone used EtBr in an auto-sequencer
non-denaturing gel to detect DNA fragments that
have no endogenous fluorescent tag?  This seems
like it should work, since the EtBr/DNA emission
wavelength is 590nm, about the same as ROX from
ABI.  I'm wondering whether a log or two of
sensitivity over agarose gels+ UV-box might be
possible.

Rob Preston
rapr at med.pitt.edu




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