pHing sodium Acetate

FJVAND00 at ukcc.uky.edu FJVAND00 at ukcc.uky.edu
Wed May 4 08:18:05 EST 1994


In article <GREENWOODP.1.0010A911 at AGRESEARCH.CRI.NZ>
GREENWOODP at AGRESEARCH.CRI.NZ (Penny Greenwood) writes:
 
>hi, We're having great difficulty getting 3M NaAc to pH 4.0, for use in a
>polyA-enriched RNA extraction procedure. If anyone has any ideas or advice on
>how to achieve this please reply to the net.
>
>Cheers Penny and happy little new netter Laurel.
 
Pain isn't it!  The trick is to start with the acetic acid and pH it UP with
the acetate.  Then all you have to do is decide what it is that you want to
be 3M (the sodium or the acetate).
 
Cheers, Frank.



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