Non-radioactive primer extension assay

[RICHARD MEINERSMANN]

Does anybody have a method for non-radioactive primer extension assay
that they can share.  I need  a method that is robust enough to work with
bacterial RNA that does not have poly-A tails and therefore is difficult to 
separate mRNA.

Thanks for any help.

Rick Meinersmann
USDA Agricultural Research Service
Athens, GA
RMeinersmann at asrr.arsusda.gov