h-seifert at nwu.edu
Thu May 5 13:57:59 EST 1994
In article <2qbafh$mlm at gazette.bcm.tmc.edu> lr035778 at mbcr.bcm.tmc.edu (Laurence T. Reiter) writes:
>I used a pre-mixed KlenTaq:pfu reagent to amplify an M13 vector (6.5 Kb) using
>a pair of rather troublesome modifying primers that enable one to use M13 in a
>shotgun seqencing protocol. It worked in all reactions on the very first try.
>Considering that I spent several weeks messing with the PCR conditions to get
>these primers to work with regular Taq Polymerase I have to say that it is well
>worth trying it.
Where did you get the 'pre-mixed' enzyme preparation?
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