Laurence T. Reiter
lr035778 at mbcr.bcm.tmc.edu
Thu May 5 12:30:25 EST 1994
I used a pre-mixed KlenTaq:pfu reagent to amplify an M13 vector (6.5 Kb) using
a pair of rather troublesome modifying primers that enable one to use M13 in a
shotgun seqencing protocol. It worked in all reactions on the very first try.
Considering that I spent several weeks messing with the PCR conditions to get
these primers to work with regular Taq Polymerase I have to say that it is well
worth trying it.
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