In vitro transcription of a large mRNA

Mike Rott mrott at unixg.ubc.ca
Fri May 6 11:38:35 EST 1994


Derek

I have routinely made in vitro viral RNA transcripts up to 8.4 Kb without
any problems using standard protocols for T7 RNA polymerase.  I read
somewhere that you can improve the percentage of full-length transcript of
large RNAs by doing the reaction at 24 instead of 37 degrees but have not
tried this. 

good luck,
 
mike



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