Need to use RNAse for RT-PCR ?

ROGER GREEN,MEDICINE,ST.JOHN'S,NF,CAN roger at kean.ucs.mun.ca
Fri May 6 14:06:22 EST 1994


	We wish to amplify (by PCR) a fragment using cDNA as template. The 
cDNA was produced by reverse-transcribing total RNA. Is there any 
neccessity to use RNAse H on the template cDNA ? How might the RNA 
interfere with the PCR reaction ?

Roger C. Green,	Faculty of Medicine               Phone: (709)737-6884
Memorial University , St. John's, Newfoundland    FAX  : (709)737-7010



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