Need to use RNAse for RT-PCR ?
roger at kean.ucs.mun.ca
Fri May 6 14:06:22 EST 1994
We wish to amplify (by PCR) a fragment using cDNA as template. The
cDNA was produced by reverse-transcribing total RNA. Is there any
neccessity to use RNAse H on the template cDNA ? How might the RNA
interfere with the PCR reaction ?
Roger C. Green, Faculty of Medicine Phone: (709)737-6884
Memorial University , St. John's, Newfoundland FAX : (709)737-7010
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