Coexpression of isotypes?
ptk at biobase.aau.dk
Fri May 6 03:44:57 EST 1994
I have cloned Ab secreting hybridomas 15 times by limiting dilution and
isotyped the Abs several times along the way. The result has always been
the same : gamma 2a, gamma 3, kappa and lambda chains. After 15 clonings
I find it difficult to believe that the cells are not monoclonal. Has
anybody ever heard of coexpression of more than one isotype? Or do you
think that they are still not clean?
Best wishes Lisbeth
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