transfections

[Martin Leach]

[unsuccessful t/fection stuff deleted]

We had the same problem with rat cerebellar granule cell cultures.

Nothing would get DNA into them.

two possiblities we havent tried are colloidal gold particle injection and
defective HSV vectors.

I believe HSV vectors could do the job (seen data from other people).

The gold injection (gold particles and dna injected at high velocity) is
another method, but never got a demo of the VERY EXPENSIVE machine.

try a defective viral vector.

martin

-- 

.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
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