leach at mbcrr.harvard.edu
Mon May 9 23:46:53 EST 1994
[unsuccessful t/fection stuff deleted]
We had the same problem with rat cerebellar granule cell cultures.
Nothing would get DNA into them.
two possiblities we havent tried are colloidal gold particle injection and
defective HSV vectors.
I believe HSV vectors could do the job (seen data from other people).
The gold injection (gold particles and dna injected at high velocity) is
another method, but never got a demo of the VERY EXPENSIVE machine.
try a defective viral vector.
..... Martin Leach Email:leach at mbcrr.harvard.edu
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