reaction mechanism for CBB

Cornelius Krasel krasel at alf.biochem.mpg.de
Tue May 10 04:32:08 EST 1994


Ding Ming (ming at ahabs.wisc.edu) wrote:
: does anyone have some idea about the reaction mechanism of Coomassie
: Brilliant Blue with proteins? If so, please post a reference. Thanks in
: advance.

I believe this has come up before. Anyways, there is this one:

S. J. Compton, Clive G. Jones (1985):
Mechanisms of Dye Response and Interference in the Bradford Protein Assay.
Anal. Biochem. 151, 369-374.

I think there is a paper in the Meth. Enz. volume about protein purification
(edited by Murray P. Deutscher, don't recall the number) about the mechanism
of CBB binding, too.

--Cornelius.

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