A grinding problem

Mon May 9 23:28:11 EST 1994

Depending on the size of your samples a simple tissue homogenizer might
work best.  These allow you to grind tissue samples with sterile tips in
sterile tubes to avoid any DNA cross-over problems that you will inevitably
run into with reusable hardware like mortars and pestels.  If your shrimps
are small enough you can avoid all grinding by doing overnight proteinase K
digestions.  I have used this technique for extracting genomic DNA from
hunks of mollusc tissue in 1.5 ml epi tubes.  This yields plenty of DNA.   

More information about the Methods mailing list