non radi detection/ blocking?

Richard Near rocket1 at bu.edu
Tue May 10 13:12:51 EST 1994


I'm trying out a non radioactive detection kit
from S&S involving biotin labeled probes and
strepaviton- alk phos -chemilum detection.  During
the incubation and right before it (the strepavit step)
the kit calls for some "blocking reagent" which of course
does not list any ingredients and is expensive.  So I
am asking if anyone has a home-made recipe for a
blocking reagent that is inexpensive, since we are
a small acad. lab. on a tight budget.

thanx
rich
rocket1 at bu.edu



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