CAT cell-per-cell detection
nick at lmb1.rug.ac.be
Wed May 11 12:02:09 EST 1994
As an essential part of my PhD investigation, I am using chloramphenicol
acetyltransferase (CAT) as a reporter gene. As I am using it in fusion with
the gene of my interest, and expressing it in a transient way, I would like to
make a distinction between cells being effectively transfected, and those who
I know that antibodies are available against CAT, which I could use
after permeabilizing the cells. However, it would be more interesting if I
could keep the membrane structure of my cells intact, and measure in one run
of the cell sorter (FACS) the activity/presence of CAT and the activity of the
gene product of interest.
Is anyone familiar with immunodetection of CAT using FACS analysis, or do
some of you know about fluorescent substrates for CAT detection.
Please post to this newsgroup, or mail me at the address given below. I'll
summarize it all, promised!
Laboratory of Molecular Biology
University of Gent
E-mail: Nick at lmb1.rug.ac.be
More information about the Methods