bl275 at cleveland.Freenet.Edu
Thu May 12 00:51:21 EST 1994
In a previous article, flavio at u.washington.edu (Flavio Solca) says:
>I am trying to use a 265 bp PCR fragment to screen a lambda gt11 library.
>Random labeling this probe works reasonably well but the fragments that
>are produced are a tad small. I hadproblems in getting a signal on my
>Does anyone know a way out?
just toss in a dash of hot nucleotide to your pcr mixture. you dont need
to adjust the cold nucleotide. i have labeled 1 kb pBR PCR fragments with
tritium, 32-P or 33-P - works lovely dude. nice high specific activity
probes in any quantity from micrograms to milligrams.
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