PCR probe

Dan Diaz bl275 at cleveland.Freenet.Edu
Thu May 12 00:51:21 EST 1994


In a previous article, flavio at u.washington.edu (Flavio Solca) says:

>Hi netters
>
>I am trying to use a 265 bp PCR fragment to screen a lambda gt11 library. 
>Random labeling this probe works reasonably well but the fragments that 
>are produced are a tad small. I hadproblems in getting a signal on my 
>filters.
>
>Does anyone know a way out?

just toss in a dash of hot nucleotide to your pcr mixture.  you dont need
to adjust the cold nucleotide.  i have labeled 1 kb pBR PCR fragments with
tritium, 32-P or 33-P - works lovely dude.  nice high specific activity
probes in any quantity from micrograms to milligrams.

diz



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